A novel covalently modified glassy carbon electrode with β-cyclodextrin was prepared via electropolymerization technique for the simultaneous determination of uric acid(UA),xanthine(XA),hypoxanthine(HX) and dopamine(DA).This new electrode presented an excellent electrocatalytic activity towards the oxidation of UA,XA,HX and DA by cyclic voltammetry(CV) method.The oxidation peaks of the four compounds were well defined and had the enhanced peak currents.The separation potentials of the oxidation peaks for DA-UA,UA-XA and XA-HX were 150,390 and 360 mV in CV,respectively.By means of differential pulse voltammetry(DPV) method,the calibration curves in the ranges of 10―225,5―105,10―170 and 5―150 μmol/L were obtained for UA,XA,HX and DA,respectively.The lowest detection limits(S/N=3) were 5,1.25,5 and 1.5 μmol/L for UA,XA,HX and DA,respectively.The practical application of the modified electrode was demonstrated by the determination of DA in hydrochloride injection and UA,XA,HX in human urine samples. A novel covalently modified glassy carbon electrode with β-cyclodextrin was prepared via electropolymerization technique for the simultaneous determination of uric acid (UA), xanthine (XA), hypoxanthine (HX) and dopamine (DA). This new electrode presented an excellent electrocatalytic activity towards the oxidation of UA, XA, HX and DA by cyclic voltammetry (CV) method. The oxidation peaks of the four compounds were well defined and had the enhanced peak currents. separation potential of the oxidation peaks for DA-UA, UA- XA and XA-HX were 150, 390 and 360 mV in CV, respectively.By means of differential pulse voltammetry (DPV) method, the calibration curves in the ranges of 10-225, 5-105, 10-170 and 5-150 μmol / The results were obtained for UA, XA, HX and DA, respectively. The lowest detection limits (S / N = 3) were 5, 1.25 and 1.5 μmol / L for UA, of the modified electrode was demonstrated by the determination of DA in hydrochloride injection and UA, XA, HX in human urine samples.