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目的探讨HPV16和HPV18感染及P53基因异常表达与大肠肿瘤发生间的关系。方法采用地高辛标记的HPV16和HPV18DNA深外分别在40例大肠癌和30例大肠腺瘤组织石蜡切片上进行原位杂交探测HPVDNA。同时，采用SABC法检测大肠癌和大肠腺瘤的P53表达水平。结果受检大肠腺瘤组织HPVDNA阳性8例（27％），其中HPV16DNA5例，HPV18DNA3例，主要见于管状绒毛状腺癌和绒毛状腺瘤；大肠腺癌组织中HPVDNA阳性19例（4％），其中HPV16DNA14例，HPV18DNA5例。HPVDNA主要见于肿瘤细胞核中，少部分见于胞浆中。大肠癌组织中HPV16．18DNA检出阳性率明显高于大肠腺癌，且腺痛中HPV16DNA阳性率明显高于其他类型腺瘤。大肠癌P53蛋白阳性率为48％；腺癌的阳性率则为16．2％。结论HPV16．18型感染并整合至宿主细胞DNA中可能导致P53基因突变与大肠腺癌发生有密切关系。
Objective To investigate the relationship between HPV16 and HPV18 infection and abnormal expression of P53 gene and colorectal tumorigenesis. Methods HPV DNA was detected by in situ hybridization on the paraffin sections of 40 cases of colorectal cancer and 30 cases of large intestine adenoma using digoxigenin-labeled HPV16 and HPV18 DNA. At the same time, the expression of P53 in colorectal cancer and colorectal adenoma was detected by SABC method. Results HPV DNA positive was detected in 8 cases (27%) of colorectal adenomas. HPV16 DNA was found in 5 cases and HPV18 DNA was found in 3 cases. It was mainly seen in tubular villous adenocarcinoma and villous adenoma. HPV DNA was positive in 19 cases (4%) in colorectal adenocarcinoma. Of these, 14 were HPV16 DNA and 5 were HPV18 DNA. HPV DNA is mainly found in the nucleus of tumors, and a small part is found in the cytoplasm. The positive rate of HPV16.18 DNA detection in colorectal cancer tissue was significantly higher than that of colorectal adenocarcinoma, and the positive rate of HPV16 DNA in adenoids was significantly higher than other types of adenomas. The positive rate of P53 protein in colorectal cancer was 48%; the positive rate of adenocarcinoma was 16.2%. Conclusion HPV16.18 infection and integration into host cell DNA may lead to a close relationship between P53 gene mutation and the occurrence of colorectal adenocarcinoma.