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目的:建立气血双补口服液中淫羊藿苷含量的测定方法,为提高该制剂的质量标准提供研究依据。方法:采用HPLC法,流动相:乙腈-纯化水(27∶73);色谱柱:汉邦ODS-C18柱(250 mm×4.6 mm,5μm);体积流量:1.0 mL/min;柱温:室温;检测波长:270 nm;进样量:10μL。结果:淫羊藿苷在55-275μg/mL范围内线性关系良好,平均回收率为99.41%,RSD%为0.94%。结论:该方法简便、可靠、重现性好,可用于气血双补口服液的质量控制。
Objective: To establish a method for the determination of icariin in Qisue Shuangfu Oral Liquid and provide the basis for improving the quality standard of the preparation. Methods: The mobile phase consisted of acetonitrile and purified water (27:73) by HPLC. The column was Hanbang ODS-C18 column (250 mm × 4.6 mm, 5 μm). The volume flow rate was 1.0 mL / ; Detection wavelength: 270 nm; injection volume: 10 μL. Results: Icariin had good linearity in the range of 55-275μg / mL with an average recovery of 99.41% and RSD% of 0.94%. Conclusion: The method is simple, reliable, reproducible, and can be used for quality control of qi and blood double oral solution.