论文部分内容阅读
原位聚合酶链反应(ISPCR)是1990年由Haase创立的，它是PCR与原位杂交两种方法的结合。该方法不仅使组织细胞内的靶基因扩增，而且不改变靶基因的定位，它集PCR的敏感性、特异性及原位杂交的定位性于一体。ISPCR包括直接法、间接法和标记引物法，近年来很多学者用此方法进行了爱滋病毒和肝炎病毒等的研究；并不断使之完善。影响ISPCR敏感性及特异性的因素较多，如标本固定的时间、蛋白酶的浓度及PCR的条件等，因此掌握好每一技术环节十分必要。
In situ Polymerase Chain Reaction (ISPCR) was founded by Haase in 1990 and is a combination of PCR and in situ hybridization. This method not only expands the target gene in the tissue cells but also does not change the target gene localization. It combines the sensitivity, specificity and localization of in situ hybridization. ISPCR includes direct method, indirect method and labeled primer method. In recent years, many scholars used this method to carry out research on HIV and hepatitis virus, and so on. There are many factors that affect the sensitivity and specificity of ISPCR, such as the time of specimen immobilization, the concentration of protease and the conditions of PCR, so it is necessary to master every technical aspect.