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目的了解携带供者抗原的第三方树突状细胞(DC)是否具有与供者源未成熟DC相似的免疫功能。方法雌性C_(57)BL/6小鼠、BALB/c小鼠和昆明小鼠分别为皮肤移植的供者、受者和第三方。将40只BALB/c小鼠分为对照组、环磷酰胺组、供者源未成熟DC组、第三方未成熟DC组、携带供者抗原第三方DC组,每组8只。后4组大鼠皮肤移植术前4d用环磷酰胺(200mg/kg)预处理,对照组同法给予等量等渗盐水。后3组术前2d用1ml相应DC悬液(1×10~7个/ml)预处理,并在术后12d重复给予1ml DC悬液(1×10~7个/ml)1次;前2组于上述2个时相点同法给予等量等渗盐水。记录各组皮片平均成活时间(MST)并于术后5d对皮片进行组织学观察。结果与对照组(16.1±3.5)d比较,供者源未成熟DC组和携带供者抗原第三方DC组小鼠移植皮片的MST明显延长,分别为(38.3±7.7)、(34.9±7.7)d(P<0.01);携带供者抗原第三方DC组与供者源未成熟DC组皮片的MST相近(P>0.05),但与第三方未成熟DC组(23.7±2.7)d比较,差异有统计学意义(P<0.05)。镜下见携带供者抗原第三方DC组移植皮片结构较清楚、排列有序,与供者源未成熟DC组情况相近。结论携带供者抗原的第三方DC与供者源未成熟DC,均可在一定程度上建立抗原特异性免疫耐受。
Objective To investigate whether third-party dendritic cells (DCs) carrying donor antigens have similar immune function to donor-derived immature DCs. Methods Female C_ (57) BL / 6 mice, BALB / c mice and Kunming mice were donor, recipient and third party respectively. Forty BALB / c mice were divided into control group, cyclophosphamide group, donor immature DC group, third party immature DC group and donor donor antigen third party DC group with 8 mice in each group. The rats in the latter four groups were pretreated with cyclophosphamide (200mg / kg) 4 days prior to skin transplantation, and the control group received the same amount of isotonic saline as the same method. The latter three groups were pretreated with 1ml DC suspension (1 × 10 ~ 7 / ml) 2d preoperatively and 1ml DC suspension (1 × 10 ~ 7 / ml) Group 2 in the above two time points with the same method of giving isotonic saline. The average survival time (MST) of each group was recorded and the histology was observed 5 days after operation. Results Compared with the control group (16.1 ± 3.5) days, the MST in the donor skin graft and the donor DC third group mice were significantly prolonged (38.3 ± 7.7, 34.9 ± 7.7, ) d (P <0.01). The MST of donor DCs in the third-party DC group was similar to that of donor-derived immature DCs group (P> 0.05), but was significantly higher than that in the third-party immature DCs group (23.7 ± 2.7) d , The difference was statistically significant (P <0.05). Microscopically, the third-party donor DCs with donor antigen showed a clearer epithelialized skin graft with an orderly arrangement similar to that of donor DCs. Conclusion The third-party DCs carrying donor antigens and donor-derived immature DCs can all establish antigen-specific immune tolerance to a certain extent.