传统人源ABO系统血型定型试剂的制备方法,存在筛选高效价的献血者较困难,红细胞无法再利用和难以批量生产等问题。为此,笔者设想选用亲合力、冷凝集素效价符合部颁标准的献浆者单采血浆,去除纤维蛋白原,用超滤技术在温和无相态变化条件下适量去除血清中的水分,保持血清中各种蛋白质量和球蛋白与白蛋白的比例,从而获得大量高效价和强亲合力的抗血清试剂。现将用超滤法制备人源性抗A试剂血清的方法及结果报告如下。 The preparation method of the blood type setting reagent of the traditional human ABO system has the problems that it is difficult to screen the high-titer blood donors, the red blood cells can not be reused, and the mass production is difficult. To this end, the author envisages the selection of avidity, cold agglutinin titer in line with the ministerial standard donor pulp plasma apheresis, fibrinogen removal, with ultrafiltration in moderate conditions without phase change under the appropriate removal of serum water, Maintains a variety of serum protein and globulin to albumin ratio, resulting in a large number of high titers and strong affinity antiserum reagent. Now using ultrafiltration method for the preparation of human anti-A reagent serum and the results are reported as follows.