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目的淋巴细胞转化试验是免疫学实验常开项目之一,也是医学研究中常用的方法,而不同方法处理往往影响试验的结果。本文采用2种常用的试验方法来处理标本,比较其对试验结果的影响。方法使用淋巴细胞培养液培养外周血后,将标本分别用不同的方法处理,一种是洗涤细胞后直接推片,一种是固定液处理后滴片,将片子进行瑞氏染色后采用形态计数法检测淋巴细胞的转化率。结果 2种方法有明显的差异,第2种方法转化率偏高,说明不同方法对试验结果有影响。结论作为实验室教学采用第一种方法较好,省时省力。
The purpose of lymphocyte transformation test is one of the immunological experiments often open project, but also the method commonly used in medical research, and different methods of treatment often affect the test results. In this paper, two kinds of commonly used test methods to deal with specimens, compare the impact of the test results. Methods After culturing peripheral blood with lymphocyte culture medium, the specimens were treated with different methods. One was to directly push the slices after the cells were washed, the other one was the fixed-solution-treated drops. After Wright’s stain, the morphological counts Method to detect lymphocyte transformation rate. The results of the two methods have obvious differences, the second method of high conversion rate, indicating that different methods have an impact on the test results. Conclusion As the first method of using laboratory teaching is better, saving time and effort.