通过甘蔗心叶的组织培养 ,研究了 11个甘蔗基因型的组织培养特性 .结果表明 ,用 2 0 0mg/L的PVP溶液浸泡外植体 (心叶 ) 2 0min ,明显降低了诱导培养过程中外植体的褐化率 ,同时大大提高了各个基因型的愈伤组织诱导率 .11个甘蔗基因型在同样的培养条件下 ,外植体的褐化率、愈伤组织的诱导率、愈伤组织的增殖率、愈伤组织的分化率都表现出显著的差异 . Tissue culture characteristics of 11 sugarcane genotypes were studied by tissue culture of sugarcane leaf.The results showed that soaking the explants (heart leaf) with 200 mg / L PVP solution for 20 min significantly reduced the induction rate The browning rate of the explants and the callus induction rate of all the genotypes were increased greatly.Under the same culture conditions, the browning rate of explants, the callus induction rate and callus Tissue proliferation rate, callus differentiation rate showed significant differences.