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目的:建立同时测定美辛唑酮红古豆醇酯栓中呋喃唑酮和吲哚美辛含量的高效液相色谱双波长分析方法。方法:采用ZORBAX Extend-C_(18)色谱柱(250 mm×4.6 mm,5μm),以乙腈和0.035 mol·L~(-1)的磷酸二氢钾水溶液(冰醋酸调节pH至3.0)为流动相进行梯度洗脱,流速1.0 ml·min~(-1),检测波长分别为364 nm和318 nm,柱温30℃,进样量20μl。结果:在选定的色谱条件下,呋喃唑酮和吲哚美辛在0.005~0.05 mg·ml~(-1)范围内线性关系良好,r=0.999 9,检测限分别为20 ng·ml~(-1)和26ng·ml~(-1),定量限分别为70 ng·ml~(-1)和90 ng·ml~(-1),平均回收率分别为99.4%(RSD=0.6%,n=9)和99.4%(RSD=0.3%,n=9)。结论:所建立的方法简便快速,专属性强,结果准确可靠,可用于美辛唑酮红古豆醇酯栓中呋喃唑酮及吲哚美辛的检测分析。
OBJECTIVE: To establish a dual-wavelength high performance liquid chromatography method for the simultaneous determination of furazolidone and indomethacin in metronidazole ketocaproin suppository. Methods: The mobile phase was eluted with acetonitrile and 0.028 mol·L -1 potassium dihydrogen phosphate (adjusted to pH 3.0 with glacial acetic acid) on a ZORBAX Extend-C_ (18) column (250 mm × 4.6 mm, 5 μm) The gradient elution was carried out at a flow rate of 1.0 ml · min ~ (-1). The detection wavelength was 364 nm and 318 nm, respectively. The column temperature was 30 ℃ and the injection volume was 20 μl. Results: Under the selected chromatographic conditions, furazolidone and indomethacin showed good linearity in the range of 0.005-0.05 mg · ml -1 with r = 0.999 9 and detection limits of 20 ng · ml ~ (- 1 and 26ng · ml -1, with the limits of quantitation of 70 ng · ml -1 and 90 ng · ml -1, respectively. The average recoveries were 99.4% (RSD = 0.6%, n = 9) and 99.4% (RSD = 0.3%, n = 9). Conclusion: The established method is simple, rapid and specific, and the results are accurate and reliable. It can be used for the detection and analysis of furazolidone and indomethacin in oxycinnate suppository.