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目的检测荷瘤鼠体内Nm23(肿瘤转移抑制因子)与RANTES(T细胞特异性趋化因子)的表达,探讨其与肿瘤发生、转移的关系。方法将B16恶性黑色素瘤细胞人工植入C57小鼠体内,待肿瘤形成后,处死,取肿瘤病变组织皮肤,用4%多聚甲醛固定、切片,作免疫组化染色。结果正常对照组与早期肿瘤Nm23与RANTES表达的比较差异显著(P<0.05),早期肿瘤与晚期肿瘤Nm23与RANTES表达的差异显著(P<0.05)。结论在B16黑色素瘤荷瘤鼠Nm23与RANTES的表达与肿瘤的发生、转移呈负相关。
Objective To detect the expression of Nm23 (tumor metastasis suppressor) and RANTES (T cell-specific chemokines) in tumor-bearing mice, and to explore its relationship with tumorigenesis and metastasis. Methods B16 malignant melanoma cells were implanted into C57 mice. After the tumors were formed, they were sacrificed and their skin lesions were removed. The tumor tissues were fixed with 4% paraformaldehyde and stained for immunohistochemical staining. Results The expression of Nm23 and RANTES were significantly different between the normal control group and the early stage tumors (P <0.05). The difference of Nm23 and RANTES between the early stage and the late stage tumors was significant (P <0.05). Conclusion The expression of Nm23 and RANTES in B16 melanoma bearing tumor mice is negatively correlated with the occurrence and metastasis of tumor.