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以7日龄刺槐实生苗的下胚轴作为外植体进行离体培养,研究不同生长调节剂、下胚轴部位和接种方式对不定芽分化的影响。结果表明:不同质量浓度的6-BA和NAA及组合对刺槐下胚轴的不定芽分化具有较大影响;不同部位的下胚轴再生不定芽的能力存在显著差异;下胚轴在培养基中的接种方式对不定芽的分化具有一定影响;下胚轴再生具有极性现象。刺槐下胚轴不定芽分化的最佳培养基为MS+6-BA1.5 mg·L-1+NAA0.05mg·L-1+3%蔗糖,最佳外植体为靠近胚根端的下胚轴部分,接种方式为水平接种,其不定芽诱导率最高,达68.52%,平均诱导不定芽数为5.56个;诱导不定芽生根的最佳培养基为MS+IBA0.20 mg·L-1+3%蔗糖,封口日光驯化1周后,以V(珍珠岩)∶V(泥炭土)∶V(蛭石)=1∶1∶1为基质移栽,生根苗经移栽成活率为100%。
The hypocotyls of 7-day-old Robinia pseudoacacia seedlings were used as explants to study the effects of different growth regulators, hypocotyls and inoculation methods on adventitious bud differentiation. The results showed that 6-BA and NAA with different concentrations had a great effect on the adventitious bud differentiation of Robinia pseudoacacia. The ability of regenerating adventitious buds in hypocotyls of different parts was significantly different. The hypocotyls in medium The inoculation method had certain influence on the adventitious bud differentiation. The hypocotyl regeneration had the polarity phenomenon. The best culture medium for adventitious bud differentiation of Robinia pseudoacacia was MS + 6-BA 1.5 mg · L -1 + NAA 0.05 mg · L -1 +3% sucrose. The best explants were the lower embryo proximal embryo Axis part, the inoculation method was horizontal inoculation, the adventitious bud induction rate was the highest (68.52%), the average number of induced adventitious buds was 5.56. The best medium for inducing adventitious buds rooting was MS + IBA 0.20 mg · L -1 + 3% sucrose, sealed daylight after 1 week of acclimation, with V (perlite): V (peat soil): V (vermiculite) = 1: 1: 1 as the substrate transplanting survival rate of rooted seedlings transplanted to 100% .