碱性条件下,H2O2氧化鲁米诺产生化学发光,酪氨酸对该体系的化学发光信号具有显著的抑制作用。据此建立了一种直接测定酪氨酸的流动注射化学发光分析新方法。该方法线性范围为4.0×10-7~2.0×10-5mol/L,检出限为1.0×10-7mol/L。对8.0×10-6mol/L的酪氨酸平行测定6次,其相对标准偏差为2.9%。该方法可应用于尿液中酪氨酸的测定。 Under alkaline conditions, luminol oxidized by H2O2 produced chemiluminescence and tyrosine had a significant inhibitory effect on the chemiluminescence signal of the system. A new method for the determination of tyrosine by flow injection chemiluminescence was established. The linear range of this method was 4.0 × 10-7 ~ 2.0 × 10-5mol / L with the detection limit of 1.0 × 10-7mol / L. The relative standard deviation was 2.9% for six parallel determinations of 8.0 × 10 -6 mol / L tyrosine. This method can be applied to the determination of tyrosine in urine.