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提出了共振瑞利散射光谱法测定洛美沙星的新方法。在pH 5.0—6.0的HAc-NaAc缓冲溶液中,铽()与洛美沙星(Lomefloxacin,LOM)抗生素能形成阳离子配合物,它可进一步通过静电引力和疏水作用与茜素红(Alizarin Red,AR)阴离子反应形成1:2:1(Tb3+:LOM:AR)三元离子缔合物,此时将出现新的共振瑞利散射(Resonance Rayleigh Scattering,RRS)光谱,其两个散射峰分别位于370nm和590nm处。在370nm处,一定浓度的洛美沙星与散射增强(ΔI)成正比,其线性范围和检出限(3σ)分别是0.004—3.52μg.mL-1和14.3 ng.mL-1。该方法简单、快速、具有良好的选择性和重复性,可用于不同体液中洛美沙星药物的测定。文中还对反应机理作了讨论。
A new method for the determination of lomefloxacin by resonance Rayleigh scattering spectrometry was proposed. Terbium and Lomefloxacin (LOM) antibiotics can form cationic complexes in HAc-NaAc buffer solution at pH 5.0-6.0, which can further interact with Alizarin Red (AR) by electrostatic attraction and hydrophobic interaction ) Anion to form a 1: 2: 1 (Tb3 +: LOM: AR) ternary ion association complex, a new Resonance Rayleigh Scattering (RRS) spectrum will appear with two scattering peaks at 370 nm And 590 nm. At 370 nm, a certain concentration of lomefloxacin was proportional to the enhancement of scattering (ΔI), and its linear range and detection limit (3σ) were 0.004-3.52 μg.mL-1 and 14.3 ng.mL-1, respectively. The method is simple, rapid, has good selectivity and repeatability and can be used for the determination of lomefloxacin in different body fluids. The article also discussed the reaction mechanism.