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柴胡为伞形科植物柴胡或狭叶柴胡的干燥根,具有镇痛、解热、抗炎之功效,常用于流感的治疗.柴胡皂苷是柴胡的主要活性成分,本研究采用高效液相色谱法,以蒸发光散射检测器进行检测,对山西不同产地野生柴胡(北柴胡)的根、茎、叶、花中柴胡皂苷a、c、d进行了含量测定,结果表明,东山、西山、天龙山、庞泉沟采集的柴胡根中3种柴胡总含量分别为4.26 mg/g,3.22 mg/g,4.23 mg/g和3.05 mg/g.然而,上述地区的柴胡茎,叶和花中几乎不含有柴胡皂苷.高效液相色谱-ELSD法适用于柴胡药材的质量控制,本研究的结果也验证了北柴胡以根入药的传统入药习惯.“,”As a traditional Chinese herbal medicine exhibiting analgesic,fever-reducing and anti-inflammatory effects,Radix Bupleuri (Chai-Hu) is commonly used for the treatment of influenza,which is derived from the dried roots of Bupleurum chinense DC.and Bupleurum scorzonerifolium Willd.Among of diverse chemical components,saikosaponins are the key active components of the herb medicine.In the present study,we established a method of high performance liquid chromatography (HPLC)coupled with evaporative light scattering detection (ELSD) for simultaneous determination of saikosaponin a,c and d in root,stem,leaf and flower of Bupleurum chinense (B chinense) collected from different areas of Shanxi Province,China.The results from 16 samples of root,stem,leaf and flower of B chinense demonstrated that the total contents of the three saikosaponins in the root of B chinense collected from Dongshan Taiyuan,Xishan,Tianlongshan and Pangquangou were 4.26 mg/g,3.22 mg/g,4.23 mg/g and 3.05 mg/g,respectively.However,there was scarcely any saikosaponins in the stem,leaf and flower of B chinense collected from above-mentioned areas.The method of HPLC coupled with ELSD was suitable for quality control of Radix Bupleuri.The result also confirmed that the root ofB chinense was the best medicinal part.