用甲酸代替药典规定的流动相中的醋酸测定庆大霉素 C组份 ,使 C1 峰与相邻杂质峰分离效果显著 ,重现性好。RSD为C1 0 .47%、C1 a0 .6 6 %、C2 +C2 a0 .5 2 %。用 C1 a标准品进行回收率和线性范围实验 ,平均回收率为 97.7%。C1 a标准品在 5 0～ 80 0μg.m l- 1范围内呈良好的线性关系 ,进一步证明该法用于庆大霉素 C组份相对比率的测定 ,精密度高 ,结果准确可靠 Determination of gentamicin component C with formic acid in place of the acetic acid in the mobile phase specified in the Pharmacopoeia results in significant separation of peak C1 from adjacent impurity peaks and good reproducibility. RSD is C1 0 .47%, C1 a0. 6 6%, C2 + C2 a0. 52%. Recoveries and linear range experiments were performed with C1 a standard with an average recovery of 97.7%. The calibration curve of C1 a showed a good linearity in the range of 50 ~ 80 μg · ml-1, which further proved that the method was applied to the determination of the relative ratio of gentamicin C component with high precision and accurate results