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研究了两个个体特征差异较大且面对干旱有不同响应机制的木薯品种SC124和Arg7,比较了在干旱处理3 d、12 d、18 d后DNA甲基化的变化情况,探究DNA甲基化在木薯抵御干旱胁迫中的作用。利用全基因组甲基化检测技术扩增片段单核苷酸多态性位点和甲基化位点(AFSM)检测基因组上的DNA甲基化水平变化情况。根据AFSM建库标准构建了测序文库,并对文库进行了质量检测。根据AFSM测序数据分析表明,木薯基因组中叶片的甲基化比例为5.9%~28.6%,根的甲基化比例为4.1%~13.5%。干旱胁迫使植株甲基化总体水平产生变化,并且具有组织和时空特异性。木薯面对干旱胁迫根据品种的不同,DNA甲基化具有不同的相应特点,且和处理时期和组织部位相关。
Two cassava cultivars SC124 and Arg7 with different characteristics and different responses to drought were studied. The changes of DNA methylation after drought treatment for 3 d, 12 d and 18 d were compared, and DNA methyl The role of tapioca in drought resistance of cassava. Genomic DNA methylation detection was used to amplify the single nucleotide polymorphisms (SNPs) and methylation sites (AFSMs) to detect DNA methylation levels in the genome. Based on the AFSM database, a sequencing library was constructed and the quality of the library was tested. According to the AFSM sequencing data, the methylation percentage of cassava genome was 5.9% -28.6% and the root methylation ratio was 4.1% ~ 13.5%. Drought stress changes the overall level of plant methylation, and has tissue and space-time specificity. Cassava Facing Drought Stress Depending on the breed, DNA methylation has different characteristics and is related to the duration of treatment and the location of the tissue.