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主要观察了重组基因工程人红细胞生成素(r-HuEPO)腹腔注射对大鼠长期毒性实验中血细胞和骨髓的刺激作用.实验分四组即溶剂对照组,生理盐水对照组,重组基因工程人红细胞生成素小剂量和大剂量组.连续给药3个月(每月测抗体1次)后,分别检测各组动物的血红蛋白、红细胞压积、血小板和骨髓等ll项指标.结果发现大.小剂量组的血红蛋白和红细胞压积均非常显著增高,骨髓中红系增生明显活跃,以早、中、晚幼有核红细胞为主,与对照组相比有显著差异(P<0.01 ),双核及核分裂多见.血小板增高,粒系增生相对受抑.停药30天后除部分骨髓象没有恢复正常外,血象指标均转为正常.
The effects of r-HuEPO intraperitoneal injection on the blood cells and bone marrow in long-term toxicity experiment in rats were mainly observed in four groups: solvent control group, saline control group, recombinant genetic engineering human erythrocyte Small doses and high-dose group, continuous administration of 3 months (measured antibody once a month), were detected in each group of hemoglobin, hematocrit, platelet and bone marrow and other 11 indicators. Hemoglobin and hematocrit of the dose group were significantly increased, erythroid hyperplasia in bone marrow was significantly active, with early, middle and late young nucleated erythrocytes, compared with the control group were significantly different (P <0.01), dual-core and More common nuclear fission thrombocytopenia, granulomatous hyperplasia relatively suppressed after 30 days withdrawal except for some bone marrow did not return to normal, the blood indicators were turned to normal.