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基于适体与目标物的高特异性识别能力,结合氧化石墨烯(GO)与单、双链DNA结合方式的差异和对荧光染料的高效猝灭特性,以SYBR Green I(SG)为荧光信号物质,溶菌酶适体(LBA)为分子识别探针,建立了一种在均相溶液中无标记检测溶菌酶的荧光分析方法。实验考察了不同互补链,SG,GO浓度,反应时间等因素对检测灵敏度的影响。在0.2~1μg/m L范围内,溶菌酶浓度与体系荧光强度变化率呈良好的线性关系,检出限0.1μg/m L。方法可应用于人血清样品中溶菌酶含量的测定。
Based on the high specific recognition ability of aptamer and target, combined with the difference between the combination of GO and single and double stranded DNA and the efficient quenching of fluorescent dye, SYBR Green I (SG) was used as fluorescence signal The LBA was used as the molecular recognition probe to establish a fluorescence-free assay for the detection of lysozyme in a homogeneous solution. The effects of different complementary strands, SG, GO concentration and reaction time on the detection sensitivity were investigated experimentally. In the range of 0.2 ~ 1μg / m L, the lysozyme concentration showed a good linear relationship with the change rate of fluorescence intensity of the system, with the detection limit of 0.1μg / m L. The method can be applied to the determination of lysozyme in human serum samples.