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MADS-box基因参与植物多种发育过程,尤其在花发育过程中发挥着重要的调控作用。该研究以玉米不育系C48-2及保持系N48-2为材料,采用RT-PCR克隆mads3基因。序列分析发现,在不育系C48-2中该基因编码区发生单碱基突变,导致其推定的蛋白质中5个氨基酸序列发生改变。荧光定量PCR显示,相对于保持系,花粉母细胞时期和四分体时期mads3基因在C48-2下调表达,而单核期和双核期上调表达。这些结果为进一步了解玉米C型细胞质雄性不育的发生机制提供参考。
The MADS-box gene is involved in many developmental processes of plants, and plays an important regulatory role especially in floral development. In this study, maize CMS line C48-2 and maintainer line N48-2 were used to clone mads3 gene by RT-PCR. Sequence analysis revealed that a single-base mutation in the coding region of C48-2 resulted in a change of 5 amino acid sequences in the putative protein. Fluorescence quantitative PCR showed that mads3 gene was downregulated in C48-2 and monocytes and dinocytes were up-regulated in pollen mother cell stage and tetrad stage relative to maintainer lines. These results provide a reference for further understanding of the mechanism of C-type cytoplasmic male sterility in maize.