为了研究Ｐ５３蛋白异常表达在结直肠腺癌患者预后中的意义，应用Ｐ５３单克隆抗体、ＡＢＣ免疫组化方法结合微波炉抗原修复法研究４０例结直肠腺癌的Ｐ５３蛋白异常表达与预后的关系。结果显示：６５％（２６／４０）的结直肠腺癌为Ｐ５３蛋白异常表达阳性。阳性病例的五年存活率为５０％（１３／２６），而阴性病例为８５７％（１２／１４），提示Ｐ５３蛋白异常表达与结直肠腺癌预后关系密切（Ｐ＜００５），可能成为一个新的预后判测指标。如结合传统的预后判测因素，可更全面地监测结直肠腺癌患者预后 In order to study the significance of abnormal expression of P53 protein in the prognosis of colorectal adenocarcinoma patients, the relationship between the abnormal expression of P53 protein and the prognosis of 40 colorectal adenocarcinomas was studied using monoclonal antibodies against P53, ABC immunohistochemistry and microwave antigen retrieval. The results showed that 65% (26/40) of colorectal adenocarcinomas were positive for abnormal expression of P53 protein. The five-year survival rate of positive cases was 50% (13/26), and negative cases were 85.7% (12/14), suggesting that the abnormal expression of P53 protein is closely related to the prognosis of colorectal adenocarcinoma (P<005). It may become a new indicator of prognosis. Combined with traditional prognostic factors, the prognosis of patients with colorectal adenocarcinoma can be more comprehensively monitored