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以２ 年生杜仲带芽茎段为起始材料进行离体快繁时，最佳取材时间是５ 月份。在初代培养中，同一枝条上中段腋芽容易萌发，茎段竖插于培养基比横放好，在一定浓度范围内，培养基中细胞分裂素与生长素浓度比为１０ ：１ 时腋芽萌发率最高。将腋芽萌发后长成的新技转入添加 Ｂ Ａ Ｐ（１ ．０ ｍ ｇ／ Ｌ） 和 Ｎ Ａ Ａ（０ ．１ ｍ ｇ／ Ｌ） 的培养基中，第二轮增殖效果较好，以后则出现玻璃化等不正常现象，只有在培养基中加入 Ａｇ Ｎ Ｏ３ 后才能使茎芽的生长恢复正常。由快繁产生的枝条经壮苗培养后转入１／４ Ｍ Ｓ＋ 间苯三酚（ Ｐ Ｇ）１０ ｍ ｇ／ Ｌ＋ Ｉ Ｂ Ａ２ ．０ ｍ ｇ／ Ｌ 培养基中，生根率较高（４３ ．０ ％ ） 。
With 2-year-old Eucommia with bud stem segment as the starting material in vitro propagation, the best time to draw is in May. In the primary culture, axillary buds were easy to germinate in the middle of the same shoots, and the stems were inserted vertically in the medium. In a certain concentration range, the ratio of cytokinin and auxin in the medium was 10: 1, highest. The new technology developed after the axillary bud was transferred into the medium supplemented with B A P (1.0 mg / L) and NAA (0.1 ml / L), the second round of proliferation was better, After that there are abnormal phenomena such as vitrification. The growth of stem buds can be restored to normal only by adding AgN03 in the medium. The shoots produced by propagation were transferred to 1/4 M S + phloroglucinol (P G) 10 m g / L + I B A2 after strong seedling culture. In 0 m g / L medium, the rooting rate was higher (43.0%).