论文部分内容阅读
采用相对荧光定量PCR( RT-PCR)相对标准曲线方法,对籽鹅和卡洛斯鹅不同产蛋时期初级卵泡、小白卵泡、中白卵泡和大白卵泡中神经生长因子( NGF) mRNA的表达量进行研究,同时检测血液中促卵泡素( FSH)的含量,并与NGFmRNA表达量进行相关性分析. 结果表明:产蛋高峰期,籽鹅初级卵泡中NGF mRNA表达量极显著高于其他卵泡( P <0. 01 ) ,卡洛斯鹅初级卵泡中 NGF mRNA表达量显著高于中白卵泡( P<0. 05) ,其余卵泡间差异不显著. 产蛋末期,籽鹅初级卵泡中NGF mRNA表达量极显著高于中白卵泡和小白卵泡( P<0. 01) ,大白卵泡NGF mRNA表达量极显著高于中白卵泡( P<0. 01) ,卡洛斯鹅初级卵泡NGF mRNA表达量极显著高于中白卵泡和小白卵泡( P<0. 01) ,大白卵泡极显著高于其他卵泡( P<0. 01). 2种鹅产蛋高峰期血液中FSH表达水平高于产蛋末期( P<0. 05);籽鹅和卡洛斯鹅的小白卵泡NGF mRNA表达量与血液中FSH的表达水平呈显著正相关( P<0. 05) ,在大白卵泡中两者呈负相关,其他卵泡呈正相关.“,”In this study, pre-hierarchical follicles of Zi geese and Carlos geese were used as materi-als. By RT-PCR technique, expression of NGF in pre-hierarchical follicles of different periods was quantitatively studied, change of follicle stimulating hormone ( FSH) in two kinds of geese blood was tested, and relationship between content of FSH and expression of NGF was explored. The results were as follows:relative expression amount of NGF in primary follicles of Zi geese is very significant-ly higher than that of other models except that of the largest white follicles, which is in peek period ( P<0. 01 ) . Expression amount of NGF in pre-hierarchical follicles of Carlos geese is higher than that of middle white follicles ( P<0. 05) . For Zi geese, during the last stage of laying, expression a-mount of NGF in pre-hierarchical follicles is very significantly higher than that of middle white folli-cles ( P<0. 01) . For Carlos geese, expression amount of NGF in large white follicles is very signifi-cantly higher than that of other models ( P<0. 01) . Expression amount of NGF in primary follicles of Zi geese peek period, the last stage of laying and Carlos geese peek period is very significantly high-er than that of the last stage of Carlos geese ( P<0. 01) . Expression amount of NGF in small white follicles in Zi geese and Carlos geese show significantly positive correlation with expression amount of FSH in serum (P<0. 05). It shows a negative correlation with expression amount of FSH. And it shows a positive correlation with expression amount of FSH.