论文部分内容阅读
目的建立高效液相色谱法(HPLC)同时测定纳豆中6种大豆异黄酮含量测定方法。方法通过考察不同的预处理方法,用正交试验设计确定最佳提取条件,优化色谱条件,建立HPLC的测定方法。色谱柱:Agilent Eclipse plus-C18(4.6mm×150mm,5μm);流动相:甲醇+0.5%乙酸梯度洗脱;流速1.0mL/min;检测波长254nm;柱温40℃。结果大豆苷、大豆黄苷、染料木苷、大豆素、大豆黄素和染料木素分别在8.2~82.0、8.3~83.0、8.0~80.0、8.2~82.0、8.6~86.0和8.0~80.0μg/mL范围内线性关系良好;检出限分别为0.037、0.034、0.028、0.019、0.026和0.025μg/mL;回收率95.5%~102.8%;测得纳豆中含有大豆苷1.35mg/g、大豆黄苷0.22mg/g、染料木苷0.96mg/g、大豆素0.17mg/g、大豆黄素0.12mg/g和染料木素0.25mg/g。结论 HPLC法快捷简便、准确可靠、灵敏度高,符合日常检测要求,为纳豆保健功能开发奠定基础。
Objective To establish a method for the simultaneous determination of six isoflavones in natto by high performance liquid chromatography (HPLC). Methods By examining different pretreatment methods, orthogonal experimental design was used to determine the optimal extraction conditions, and the chromatographic conditions were optimized. The HPLC determination method was established. Column: Agilent Eclipse plus-C18 (4.6mm × 150mm, 5μm); mobile phase: methanol + 0.5% acetic acid gradient elution; flow rate 1.0mL / min; detection wavelength 254nm; Results Daidzein, daidzin, genistin, daidzein, daidzein and genistein were in the range of 8.2 ~ 82.0, 8.3 ~ 83.0, 8.0 ~ 80.0, 8.2 ~ 82.0, 8.6 ~ 86.0 and 8.0 ~ 80.0 μg / mL The linearity was good. The detection limits were 0.037,0.034,0.028,0.019,0.026 and 0.025μg / mL, respectively. The recoveries ranged from 95.5% to 102.8%. The results showed that natto contains 1.35mg / g daidzin, 0.22mg daidzein, / g, genistin 0.96 mg / g, daidzein 0.17 mg / g, daidzein 0.12 mg / g and genistein 0.25 mg / g. Conclusion HPLC method is fast, simple, accurate, reliable and sensitive. It meets the requirements of daily testing and lays the foundation for the development of natto health function.