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目的:观察网织血小板(RP)在不同血液系统疾病血小板减少程度一致情况下的水平,初步探讨其对血小板减少原因判断的价值。方法:利用免疫荧光标记通过流式细胞仪检测15例健康志愿者、11例初治的特发性血小板减少性紫癜(ITP)患者、10例再生障碍性贫血患者、10例造血干细胞移植后尚未获得完全造血重建的血小板减少患者、5例造血干细胞移植获得造血重建后血小板再次减少患者的外周血网织血小板百分率。结果:初治的ITP组及造血干细胞移植获得造血重建后血小板再次减少组RP%明显高于正常对照组(P<0.01),再障组、造血干细胞移植后未获得完全造血重建的血小板减少组与正常对照组之间RP%差异无统计学意义(P>0.05)。结论:RP对鉴别血小板减少原因,尤其是免疫因素引起的血小板减少有重要意义,对临床使用免疫抑制剂治疗有指导意义。
Objective: To observe the level of reticulocyte platelets (RP) in the same degree of thrombocytopenia in different hematological diseases, and to explore the value of reticulated platelet (LP) in judging the cause of thrombocytopenia. Methods: Fifteen healthy volunteers were detected by flow cytometry with immunofluorescence staining, 11 patients with idiopathic thrombocytopenic purpura (ITP), 10 patients with aplastic anemia and 10 patients without hematopoietic stem cell transplantation To obtain a complete hematopoietic reconstructive thrombocytopenia patients, 5 cases of hematopoietic stem cell transplantation to obtain hematopoietic reconstructive platelets again reduce the percentage of patients with peripheral blood platelet reticulated platelets. Results: The RP% of the newly treated ITP group and the hematopoietic stem cell transplant recipients after hematopoietic reconstitution was significantly lower than that of the normal control group (P <0.01). However, no complete hematopoietic reconstructive thrombocytopenia group There was no significant difference in RP% with normal control group (P> 0.05). Conclusion: RP is of great importance in identifying the causes of thrombocytopenia, especially thrombocytopenia caused by immune factors, and is of guiding significance for the clinical use of immunosuppressive agents.