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目的:研究微纳米形貌二氧化钛膜的体外生物学性能,旨在寻找适合牙种植颈部软组织附着的钛表面微结构。方法:依据阳极氧化电压分为150V、180V、200V组,及机械抛光组;BCA法测定材料表面蛋白吸附;通过变形链球菌粘附和L929细胞粘附能力进行评价。结果:检测结果为机械抛光组呈浅的微沟纹,等向排列,表面光滑;阳极氧化组表面粗糙,呈椭圆形微孔,大小分布不一,直径约10 nm～2μm,孔间隔为半圆形突起,突起直径为100-500 nm。BSA吸附量,180V组吸附牛血清白蛋白量最大;变形链球菌菌悬液OD值提示各组间均存在显著性差异(p<0.05),其中180V组和200V组间差异最大,180V组菌悬液细菌量最少;L929细胞在各组钛片表面生长良好,各组钛片对细胞没有明显的毒性。cck-8测量值提示,180V组和200V组表面细胞粘附量均大于其它组(p<0.05)。讨论:钛表面的微纳米结构设计是构建种植体颈部生物学宽度、类天然牙龈牙附着的结构基础。结论:阳极氧化180V组表面形成微纳米结构的二氧化钛膜促进牛血清白蛋白的吸附,抑制细菌的粘附同时增强了成纤维细胞的粘附,对生物学封闭的形成具有积极的影响。
OBJECTIVE: To study the in vitro biological properties of titanium dioxide film with micro-nano topography, aiming to find the titanium surface micro-structure that is suitable for soft tissue attachment of dental implants. Methods: The anodic oxidation voltage was divided into 150V, 180V, 200V group and mechanical polishing group. BCA method was used to determine the surface protein adsorption. The adhesion of Streptococcus mutans and L929 cell adhesion ability were evaluated. Results: The results showed that the mechanical polishing group was shallow microfluidic groove with isotropic alignment and smooth surface. The surface of the anodic oxidation group was rough with oval micropores with different size distribution. The diameter was about 10 nm ~ 2 μm and the hole spacing was half Round protrusions with a protrusion diameter of 100-500 nm. BSA adsorption amount, 180V group had the largest amount of bovine serum albumin adsorption; OD value of Streptococcus mutans suspension suggested significant differences (p <0.05), among 180V group and 200V group, L929 cells in each group grew well on the surface of titanium, the group of titanium tablets on cells without significant toxicity. The results of cck-8 suggested that the cell adhesion on the surface of 180V and 200V groups were greater than that of other groups (p <0.05). Discussion: The design of the micro-nano structure of the titanium surface is the structural basis for the construction of the biological width of the neck of the implant and the attachment of the natural gingival teeth. CONCLUSION: Anodic oxidation of titanium dioxide film on the surface of 180V group with micro-nano structure promotes the adsorption of bovine serum albumin, inhibits the adhesion of bacteria and enhances the adhesion of fibroblasts, which has a positive effect on the formation of biological blocking.