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骨骼肌组成特征是鱼类肉质性状评价的重要基础。为全面了解鳜(Siniperca chuatsi)肉质性状的组成特征,通过制作骨骼肌石蜡切片,比较了鳜快肌和慢肌两种类型骨骼肌的组织学特征,快肌和慢肌在肌纤维细胞直径、形态上存在差异。利用SDS-聚丙烯酰胺凝胶电泳(SDS-PAGE)分析了鳜快肌和慢肌的蛋白质组成特征,它们在50～60 ku和10～15 ku大小的条带中存在差异。克隆了鳜快肌和慢肌肌球蛋白重链(MHC)S1结构域的序列,快肌和慢肌S1结构域cDNA序列长度分别为2 523 bp、2 520 bp,编码841、840个氨基酸,氨基酸序列的相似度为87.6%,以两个loop区的差异最大。研究结果表明,鳜快肌、慢肌在肌纤维细胞形态、蛋白质组成以及肌球蛋白重链分子结构上均存在较明显的差异,这些差异与其担负不同的运动功能相关。
Skeletal muscle composition is an important basis for the evaluation of meat quality traits. In order to fully understand the compositional characteristics of meat quality traits in Siniperca chuatsi, histological features of skeletal muscle of Siniperca chuatsi were compared by two types of skeletal muscle, including fast muscle and slow muscle. There are differences. Protein composition of the fast and slow muscles of the shrews were analyzed by SDS-polyacrylamide gel electrophoresis (SDS-PAGE), and they were different in bands of 50-60 ku and 10-15 ku. The sequences of S1 domain of fast muscle and slow muscle myosin heavy chain (MHC) were cloned. The length of cDNA sequence of fast and slow muscle S1 domain were respectively 2 523 bp and 2 520 bp encoding 841,840 amino acids, The amino acid sequence similarity was 87.6%, with the largest difference between the two loop regions. The results show that the fast muscle and slow muscle in muscle fiber morphology, protein composition and structure of myosin heavy chain molecules are more obvious differences, these differences and their different sports functions.