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目的探讨黄芩苷抑制人结肠癌细胞(HCT116)增殖的分子机制。方法采用MTT比色法检测不同浓度的黄芩苷(0.01～100μg/ml)对体外培养的HCT116细胞增殖的抑制作用;采用Western blot分析检测被黄芩苷干预的HCT 116细胞的增殖相关蛋白的表达。结果与对照组比较,浓度为1～100μg/ml的黄芩苷处理组明显抑制HCT 116细胞的增殖(P<0.05)。用100μg/ml黄芩苷处理后,增殖相关的蛋白mTOR、p70S6K、S6、eIF4E表达明显下调(P<0.05),4E-BP1表达明显上调。p-mTOR(Ser2448)、p-S6(Ser235/236)、p-4E-BP1(Thr37/46)的磷酸化水平下降(P<0.05)。结论黄芩苷能通过抑制mTOR信号通路来抑制人结直肠癌HCT116细胞的增殖。
Objective To investigate the molecular mechanism of baicalin inhibiting the proliferation of human colon cancer cells (HCT116). Methods The inhibitory effects of different concentrations of baicalin (0.01-100 μg / ml) on the proliferation of HCT116 cells in vitro were detected by MTT colorimetric assay. The expression of proliferation related proteins in HCT 116 cells treated with baicalin was detected by Western blot. Results Compared with the control group, baicalin at a concentration of 1 ~ 100μg / ml significantly inhibited the proliferation of HCT 116 cells (P <0.05). After treatment with 100μg / ml baicalin, the expression of mTOR, p70S6K, S6 and eIF4E were significantly down-regulated (P <0.05), and the expression of 4E-BP1 was significantly up-regulated. Phosphorylation levels of p-mTOR (Ser2448), p-S6 (Ser235 / 236) and p-4E-BP1 (Thr37 / 46) decreased (P <0.05). Conclusion Baicalin can inhibit the proliferation of human colorectal cancer HCT116 cells by inhibiting the mTOR signaling pathway.