论文部分内容阅读
本文推荐一个用荧光法测定配合物解离常数的实验 ,实验原理简单 ,知识内容跨学科 ,兼有生物化学和化学知识 ,有利于生化专业和化学专业的学生知识面的拓宽。1 实验目的 ( 1)掌握用荧光法测定配合物解离常数的原理和方法 ; ( 2 )熟悉荧光光度计的使用。2 实验原理
In this paper, we propose an experiment to determine the dissociation constant of complex by fluorescence method. The experimental principle is simple, the content of knowledge is interdisciplinary and both biochemistry and chemical knowledge are beneficial to broaden the students’ knowledge of biochemistry and chemistry specialty. 1 Experimental purposes (1) master the principle and method of determination of dissociation constant of complex by fluorescence method; (2) familiar with the use of fluorophotometer. 2 experimental principle