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文章简介虽然多重耐药菌严重危害人们的健康和动物养殖,但目前尚不清楚能够有效控制多重耐药菌感染的药物。本研究采用基于GC/MS代谢组学的研究思路和技术方法,发现卡那霉素耐药迟缓爱德华菌的葡萄糖和丙氨酸显著下调。外源性丙氨酸和葡萄糖可以重建多重耐药迟缓爱德华菌对卡那霉素杀菌作用的敏感性,从而创建了一种有效杀死多重耐药菌的新途径。该机制为外源性葡萄糖和丙氨酸通过底物激活,促进三羧酸循环,增加NADH和质子动力势,提高抗生素的摄取。这种防治耐药菌的方法在多种革兰氏阴性菌(副溶血弧菌、肺炎克雷伯菌、铜
Introduction to the article Although multiple drug-resistant bacteria seriously endanger human health and animal culture, it is not yet clear what can effectively control multi-drug-resistant infections. In this study, based on GC / MS metabolomics research ideas and technical methods, we found that kanamycin resistant Edwards can significantly degrade glucose and alanine. Exogenous alanine and glucose can reconstruct the susceptibility of multidrug-resistant Edardin bacteria to the kanamycin bactericidal effect, creating a new way to effectively kill multi-drug resistant bacteria. The mechanism of exogenous glucose and alanine through the substrate activation, promote the cycle of tricarboxylic acid, increase NADH and proton motility, improve the uptake of antibiotics. This method of prevention and treatment of drug-resistant bacteria in a variety of Gram-negative bacteria (Vibrio parahaemolyticus, Klebsiella pneumoniae, copper