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目的:检测Nectin-4在卵巢癌中的表达情况,分析其与卵巢癌临床病理参数之间的关系。方法:RT-PCR法检测30例卵巢癌、25例卵巢良性肿瘤和30例正常卵巢组织Nectin-4 mRNA表达;Western blotting法检测Nectin-4蛋白表达。结果:①Nectin-4 mRNA表达为卵巢癌组0.71±0.12,卵巢良性肿瘤组0.32±0.09、正常卵巢组0.30±0.09,卵巢癌组显著高于后两组,差异有统计学意义(P<0.01)。卵巢癌组织中,不同年龄、病理类型间比较,差异均无统计学意义(P>0.05);不同病理分化程度、手术病理分期、有无淋巴结转移比较,差异均有统计学意义(P<0.05)。②Nectin-4蛋白表达为卵巢癌组0.71±0.06、卵巢良性肿瘤组0.35±0.07、正常卵巢组0.32±0.07,卵巢癌组织明显高于卵巢良性肿瘤和正常卵巢组织,差异有统计学意义(P<0.01)。结论:卵巢癌存在Nectin-4过度表达,与其病理分化程度、手术病理分期及淋巴结转移有密切关系,提示Nectin-4参与调控卵巢癌发生及发展过程。
OBJECTIVE: To detect the expression of nectin-4 in ovarian cancer and to analyze its relationship with clinicopathological parameters of ovarian cancer. Methods: Nectin-4 mRNA was detected by RT-PCR in 30 cases of ovarian cancer, 25 cases of benign ovarian tumor and 30 cases of normal ovarian tissue. The expression of Nectin-4 protein was detected by Western blotting. Results: ①Nectin-4 mRNA expression in ovarian cancer group was 0.71 ± 0.12, ovarian benign tumor group was 0.32 ± 0.09, normal ovary group was 0.30 ± 0.09, ovarian cancer group was significantly higher than the latter two groups, the difference was statistically significant (P0.01) . There was no significant difference in ovarian cancer tissues between different age and pathological types (P> 0.05). There were significant differences in the degree of pathological differentiation, pathological stage and lymph node metastasis (P <0.05) ). The expression of nectin-4 protein was 0.71 ± 0.06 in ovarian cancer group, 0.35 ± 0.07 in benign ovarian tumor group and 0.32 ± 0.07 in normal ovarian group, and was significantly higher in ovarian cancer than in benign ovarian tumor and normal ovarian tissue (P < 0.01). Conclusion: Nectin-4 overexpression exists in ovarian cancer, which is closely related to the degree of pathological differentiation, pathological stage and lymph node metastasis. It suggests that Nectin-4 is involved in the regulation of ovarian carcinogenesis and development.