本文将正交函数分光光度法和茚三酮比色法联用测定混合氨基酸溶液中苯丙氨酸(Phenylalanine,Phe)和天门冬氨酸(Aspartate,Asp)的含量。选用一次正交多项式为特征多项武,波长区间为263～269nm,△λ=2nm的4点法测定Phe的含量,再用比色法测定混合氨基酸的总量,减去Phe的量,可得Asp的量。其平均回收率分别为99.9%,99.3%,变异系数分别为0.5%,0.7%. In this paper, the content of Phenylalanine (Phe) and Aspartate (Asp) in mixed amino acid solution was determined by orthogonal function spectrophotometry and ninhydrin colorimetry. Select an orthogonal polynomial for the characteristics of a number of weapons, the wavelength range of 263 ~ 269nm, △ λ = 2nm 4-point method for the determination of Phe content, and then mixed colorimetric determination of the total amount of amino acids, minus the amount of Phe, The amount of Asp. The average recoveries were 99.9% and 99.3%, respectively. The coefficients of variation were 0.5% and 0.7% respectively.