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目的:探讨模拟深埋条件下低氧复合缺水缺食大鼠的生命耐受力及其重要脏器血清生化指标变化规律。方法:Wistar大鼠200只,雌雄各半,随机分为对照组(40只)、单纯低氧组(40只)、缺水缺食组(60只)、低氧复合缺水缺食组(60只)。生命耐受力实验120只,观察至缺水缺食组和低氧复合缺水缺食组大鼠全部死亡,血清生化检测实验80只,于致伤后第1天、第3天、第5天、第7天检测静脉血血糖(GLU),尿素氮(BUN),肌酐(Cre),谷丙转氨酶(ALT),总蛋白(TP),白蛋白(ALB),球蛋白(GLB),碱性磷酸酶(ALP),谷草转氨酶(AST),乳酸脱氢酶(LDH),肌酸激酶(CK),以及钠(Na~+)和钙离子(Ca~(2+))。结果结果:(1)低氧复合缺水缺食组大鼠死亡时间(6.8~12.1)天较缺水缺食组死亡时间(7.8~13.4)天提前,雄性大鼠平均活存时间较雌性缩短1.6天,缺水缺食组呈同样趋势;(2)低氧复合缺水缺食组13项血清生化指标均发生变化,较低氧组和缺水缺食组呈加重趋势,其中第5~7天时AST、ALT和BUN升高和GLU降低等变化尤为严重,雄性较雌性明显。结论:(1)模拟深埋条件下低氧复合缺水缺食大鼠的生命耐受时间为7~13天,雄性平均活存时间较雌性缩短;(2)低氧复合缺水缺食组可导致心、肝、肾功能损害,较低氧和缺水缺食具有加重效应,并具有多器官性、缓慢进行性、时相差异性、性别差异性特点。
Objective: To investigate the changes of life tolerance and serum biochemical indexes of hypoxia rats under hypoxia combined with simulated water immersion. Methods: 200 Wistar rats were randomly divided into control group (40 rats), hypoxia group (40 rats), water deficit diet group (60 rats), hypoxia water deficit diet group 60). Life tolerance test of 120, observed to water deficit group and hypoxia water deficit group rats died, serum biochemical test 80, on the first day after injury, the first 3 days, 5 Day, blood glucose (GLU), blood urea nitrogen (BUN), Cre, ALT, TP, ALB, GLB, ALP, AST, LDH, CK, Na ~ + and Ca ~ (2+) were measured. Results: (1) The death time (6.8-12.1) days in hypoxic-anoxic and water-deficient diet-deficient group was earlier than that in water-deficient diet-deficient group (7.8-13.4 days), and the average survival time in male rats was shorter than that in female 1.6 days, the same trend was observed in the water-deficit food-deficient group; (3) The serum biochemical indexes of 13 items in the water-deficient food-deficient group were all changed after hypoxia, The change of AST, ALT and BUN and the decrease of GLU were especially serious on the 7th day, the male was more obvious than the female. Conclusions: (1) The survival time of hypoxia rats with hypoxia combined with water deficit under simulated deep-sea conditions is 7 to 13 days, and the average survival time in male is shorter than that in female. (2) Can lead to heart, liver and kidney dysfunction, lower oxygen and lack of water and lack of food have an aggravating effect, and with multiple organ, slow progress, time difference, gender differences.