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目的建立乙型肝炎(乙肝)病毒标志参比系统。方法选择不适合临床使用的血浆155份为预备参比品,使用美国Abbott公司生产的微粒子酶免疫测定法乙肝五项检测试剂,重复检测每份预备参比品各2次。凡2次检测结果同为阳性或阴性者,入选参比系统;凡检测结果为一阴一阳者,剔除。结果乙肝病毒表面抗原参比系统有153份血浆入选;抗乙肝病毒表面抗原抗体、抗乙肝病毒核心抗原抗体、抗乙肝病毒e抗原抗体各有154份血浆入选;乙肝病毒e抗原因只有4份阳性血浆,不能构成参比系统。结论通过使用性能出众的试剂2次重复检测,确定足够数量的阳性和阴性参比品,可以构成参比系统。使用参比系统要注意其适用范围,避免产生错误。
Objective To establish a hepatitis B (hepatitis B) virus marker reference system. Methods 155 samples of plasma which were not suitable for clinical use were selected as the reference materials. Five samples of HBsAg were tested by Abbott, USA. Where 2 test results with the same positive or negative who selected reference system; where the test results for a yin and yang, removed. Results A total of 153 plasma samples were obtained from the reference system of hepatitis B virus surface antigen. Anti-hepatitis B virus surface antigen antibody, anti-hepatitis B virus core antigen antibody and anti-hepatitis B virus e antigen antibody were selected from 154 plasma respectively. Only 4 positive Plasma, can not constitute a reference system. Conclusion The reference system can be constructed by using two replicates of a superior reagent to determine a sufficient number of positive and negative reference materials. Use the reference system to pay attention to its scope, to avoid errors.