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目的探讨应用间期荧光原位杂交(FISH)技术检测7号染色体/7号染色体长臂缺失(-7/7q-)异常在骨髓增生异常综合征(MDS)中的价值。方法采用常规细胞遗传学(CC)和间期FISH检测技术对72例MDS患者和8例正常对照者的骨髓细胞进行分析,比较CC和FISH检测-7/7q-异常的情况。结果 72例MDS中,12例CC检测阳性,发生率16.7%。间期FISH检测24例阳性,发生率33.3%。两种方法检出率有统计学差异(P<0.05)。结论间期FISH技术敏感性高于CC,并能发现和纠正CC分析中的漏检异常。
Objective To investigate the value of interphase fluorescence in situ hybridization (FISH) in detecting the abnormality of long arm (-7 / 7q-) on chromosome 7 / chromosome 7 in myelodysplastic syndrome (MDS). Methods Bone marrow cells of 72 patients with MDS and 8 normal controls were analyzed by conventional cytogenetics (CC) and interphase FISH to compare the detection of -7 / 7q-abnormalities by CC and FISH. Results In 72 cases of MDS, 12 cases of CC test positive, the incidence rate of 16.7%. Interphase FISH 24 cases were positive, the incidence of 33.3%. The detection rate of the two methods was statistically significant (P <0.05). Conclusion The interphase FISH technique is more sensitive than CC and can detect and correct undetected anomalies in CC analysis.