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目的:探讨脂联素(APN)水平及其单核苷酸基因多态性(SNP)+45T/G和+276G/T 2个位点与冠心病(CHD)的关系。方法:将195例研究对象分为正常对照组和CHD组,采用实验-对照研究方法,应用酶联免疫吸附法(ELISA)及聚合酶链反应-限制性片段长度多态性(PCR-RFLP)技术,对血浆APN浓度及APN 2个位点基因多态性进行检测。结果:2组比较,CHD组APN降低(P<0.05);CHD组SNP+45TG+GG基因型和G等位基因的分布频率明显低于正常对照组,差异具有统计学意义(P<0.05);基因SNP+276多态性位点各基因型频率与等位基因频率在2组之间差异均无统计学意义(P>0.05)。结论:CHD患者APN水平下降,SNP+45G等位基因可能为CHD的保护因素;SNP+276位点基因多态性可能与CHD的发生无明显相关性。
Objective: To investigate the relationship between adiponectin (APN) level and single nucleotide polymorphisms (SNP) + 45T / G and + 276G / T 2 loci and coronary heart disease (CHD). Methods: One hundred and ninety-five subjects were divided into normal control group and CHD group. The levels of serum lipids in the CHD group and the control group were determined by enzyme-linked immunosorbent assay (ELISA) and polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) Technology, the plasma APN concentration and APN 2 locus gene polymorphisms were detected. Results: The APN of CHD group was significantly lower than that of CHD group (P <0.05). The frequencies of SNP + 45TG + GG genotype and G allele in CHD group were significantly lower than those in normal control group (P <0.05) There was no significant difference in genotype frequency and allele frequency between the SNP + 276 polymorphism loci in the two groups (P> 0.05). CONCLUSION: The level of APN in CHD patients is decreased. The SNP + 45G allele may be the protective factor of CHD. The SNP + 276 locus polymorphism may not be associated with the occurrence of CHD.