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目的:探讨双半乳糖基二酰甘油酯(digalactosyl diglyceride,DGDG)作为一种乳化剂使用的可行性。方法:以香叶油为模型药物,油中乳化法制备初乳,通过单因素考察优化制备工艺,采用星点设计-效应面法(central composite design-re-sponse surface methodology,CCD-RSM)对处方进行优化,并对其稳定性进行初步研究。结果:确定的最终制备工艺为,60℃,油中乳化法制备初乳,于组织匀浆机中匀浆10 min,高压均质于80 Pa下循环10次,0.22μm微孔滤膜滤过灭菌,充氮灌封即得。最优处方为:DGDG用量1.6%;大豆油用量1.1%;油酸钠用量0.16%。其中香叶油用量为0.3%。所得亚微乳外观细腻洁白,所制3批样品粒径为168.0~169.3 nm,Zeta电位-25.53~24.90 mV,pH8.48~8.52,最佳处方的验证结果与预测值相差1.8%。在高温、光照条件下稳定性良好。结论:DGDG可作为香叶油亚微乳的乳化剂使用。
Objective: To investigate the feasibility of digalactosyl diglyceride (DGDG) as an emulsifier. Methods: The gels were prepared by using geranium oil as the model drug and emulsified in oil. The single factor was used to optimize the preparation process. The central composite design-re-sponse surface methodology (CCD-RSM) Prescriptions were optimized, and the stability of a preliminary study. Results: The final preparation process was as follows: colostrum was prepared by emulsification in oil at 60 ℃, homogenized in homogenizer for 10 min, homogenized under high pressure at 80 Pa for 10 times and filtered through 0.22 μm microporous membrane Sterilization, nitrogen filling pottery that. The optimal prescription is: DGDG dosage 1.6%; soybean oil 1.1%; sodium oleate 0.16%. One of the amount of gerani oil 0.3%. The obtained submicroemulsions were delicate and white in appearance. The particle size of the three batches of samples was 168.0-169.3 nm, Zeta potential -25.53-24.90 mV and pH 8.48-8.52. The validation results of the best prescriptions were 1.8% different from the predicted values. In high temperature, light conditions, good stability. Conclusion: DGDG can be used as an emulsifier for submerged microemulsion.