以大田生长的双低油菜品系“984 1”为供试材料 ,研究了影响小孢子培养反应的因素。结果表明 :始花期前、后一周的主轴花序为起始材料经 1～ 2d4℃低温预处理 ,B5培养基为提取液游离小孢子培养于含脯氨酸 2 0 0mg/L、谷氨酰胺 80 0mg/L、蔗糖 13%的NLN培养基 ,32℃热激1d后转入 2 5℃常温静置培养 ,二周后进行 4 5rpm振荡培养 ,可提高胚状体的诱导频率及正常植株再生率。 The low-rapeseed cultivar “984 1” grown in the field was used as the test material to study the factors affecting the microspore culture response. The results showed that the spindle inflorescences before and after the flowering stage were pretreated with 1 ~ 2d4 ℃ hypothermia, and the B5 medium was isolated microspore culture medium containing proline 200 mg / L, glutamine 80 0mg / L, sucrose 13% NLN medium, heat shocked at 32 ℃ for 1d and then transferred to stationary culture at 25 ℃. After two weeks, shaking culture at 45rpm, the induction frequency and normal plant regeneration rate .