【摘 要】
：
过氧化物酶在植物的损伤愈合中起着重要作用.我们采用生物化学分析和组织定位技术,确定了杜仲(Eucommia ulmoides Oliv.)剥皮再生过程中形成层区域过氧化物酶的分布和活性变
【机 构】
：
北京大学生命科学学院,High Commission of Atomic Energy/Regional Center of Nuclear Research
论文部分内容阅读
过氧化物酶在植物的损伤愈合中起着重要作用.我们采用生物化学分析和组织定位技术,确定了杜仲(Eucommia ulmoides Oliv.)剥皮再生过程中形成层区域过氧化物酶的分布和活性变化.剥皮后第4天至第21天,剥皮植株过氧化物酶含量大幅度增加(与对照比,增加了30～40倍),在整个剥皮再生过程中(从第0天至第63天),过氧化物酶在细胞分裂活跃的形成层和木栓形成层细胞中分布较少.而在愈伤组织、类皮层、成熟韧皮部和成熟木质部中分布较多.过氧化物酶在形成层及其两侧呈梯度分布,形成层中最少,在两侧的韧皮部和木质部中依次递增.其等电聚焦电泳显示两条酶带:POD Ⅰ和PODⅡ.前者只在木质部中分布,可能与木质部的分化有关;后者只在再生的组织中分布,可能与树皮再生过程有关.
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