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巴黎Gustave Roussy研究所的Georges Barski,Serge Sorieul和FrancineCornefert的新发现促进了这项工作,Barski小组研究由某个小鼠成纤维细胞而来的小鼠癌细胞系,2个细胞系在组织培养中表现出不同的表型,即染色体构型,及肿瘤形成的能力:“高癌”系(NI)容易产生肿瘤,而“低癌”系(N2)则相当弱。Barski,Sorieul和Cornefert为了能在2个细胞系发现象肺炎双球菌那样的转化,他们于1959年12月9日开始了一系列实验,将2种细胞类型(N1和N2)混合培养。经过3个月的培养,他们发现了1种出乎意料的新的细胞类型,截然与2个亲本不同,在混合培养中长得很好。看来新细胞是N1和N2细胞融合形成1个在核中有2种细胞染色体的杂种细胞。其染色体数大致是N1和N2的总和,每一系染
The new discoveries of Georges Barski, Serge Sorieul and Francine Cornefert at the Gustave Roussy Institute in Paris promoted this work. The Barski group studied mouse cancer cell lines derived from certain mouse fibroblasts and two cell lines in tissue culture. Different phenotypes, namely chromosome configuration, and tumor formation ability were observed: “High cancer” lines (NI) are prone to tumors, and “low cancer” lines (N2) are quite weak. Barski, Sorieul, and Cornefert started a series of experiments on December 9, 1959 to find two cell types (N1 and N2) in order to find that they could be transformed like pneumococci in two cell lines. After three months of training, they discovered an unexpected new cell type, distinct from the two parents, and grew well in mixed culture. It appears that the new cell is a fusion of N1 and N2 cells to form a hybrid cell with 2 cell chromosomes in the nucleus. The chromosome number is roughly the sum of N1 and N2, and each line is dyed