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目的 :观察在高浓度葡萄糖条件下体外培养兔视网膜 Müller细胞碱性成纤维细胞生长因子 (basicfibroblast growth factor,b FGF)的表达及其钙 (Ca2 + )通道的变化。方法 :采用免疫细胞化学及透射电镜方法鉴定体外培养的兔视网膜 Müller细胞 ;应用免疫细胞化学方法定性观察高糖条件下视网膜 Müller细胞 b FGF表达的变化 ;利用膜片钳技术观察高糖条件下 Müller细胞 Ca2 + 通道的变化。结果 :高糖可以刺激视网膜 Müller细胞表达b FGF,对 Ca2 + 通道无明显影响。结论 :高糖刺激 Müller细胞 b FGF的表达 ,而发挥促血管生成的作用 ,在此过程中 Ca2 + 通道无明显变化
OBJECTIVE: To observe the expression of basic fibroblast growth factor (bFGF) and the change of calcium (Ca2 +) channel in cultured rabbit retinal Müller cells under high glucose. Methods: Rabbit retinal Müller cells were identified by immunocytochemistry and transmission electron microscopy. The immunofluorescence staining was used to observe the changes of b FGF expression in retinal Müller cells under high glucose conditions. The effects of Müller Changes in cellular Ca2 + channels. Results: High glucose could stimulate retinal Müller cells to express b FGF, but had no obvious effect on Ca2 + channel. Conclusion: High glucose can stimulate the expression of b FGF in Müller cells and exert the pro-angiogenic effect. There is no significant change in Ca2 + channels during this process