论文部分内容阅读
目的研究氯化镧(lanthanum chloride,LaCl3)对原代培养的星形胶质细胞的氧化损伤作用。方法原代培养的星形胶质细胞经0、0.25、0.5、1.0 mmol/L LaCl3处理24 h后,采用MTT法和Alamar Blue还原法检测星形胶质细胞的存活率,并测定星形胶质细胞中谷胱甘肽(glutathione,GSH)、丙二醛(malondialdehyde,MDA)含量和谷胱甘肽过氧化物酶(glutathione peroxydase,GSH-Px)活性。结果各LaCl3处理组星形胶质细胞的存活率均显著低于对照组,且具有一定的剂量-反应关系。0.25 mmol/L LaCl3组星形胶质细胞的GSH含量和GSH-Px活性显著低于对照组;0.5 mmol/L LaCl3组星形胶质细胞的GSH含量和GSH-Px活性显著低于对照组和0.25 mmol/L LaCl3组;1.0mmol/LLaCl3组星形胶质细胞的GSH含量和GSH-Px活性分别降低至对照组的66%和43%,均显著低于对照组、0.25和0.5 mmol/L LaCl3组。此外,各LaCl3组星形胶质细胞的MDA含量均显著高于对照组,且随LaCl3染毒剂量增加而增加。结论LaCl3对星形胶质细胞具有损伤作用,其机制可能与LaCl3导致星形胶质细胞氧化损伤有关。
Objective To study the oxidative damage of lanthanum chloride (LaCl3) on cultured primary cultured astrocytes. Methods Primary cultured astrocytes were treated with 0, 0.25, 0.5 and 1.0 mmol / L LaCl3 for 24 h. The viability of astrocytes was detected by MTT assay and Alamar Blue reduction assay. The astrocytes Glutathione (GSH), malondialdehyde (MDA) and glutathione peroxidase (GSH-Px) activity in the stromal cells were measured. Results The viability of astrocytes in each LaCl3-treated group was significantly lower than that in the control group, and had a dose-response relationship. GSH content and GSH-Px activity of astrocytes in 0.25 mmol / L LaCl3 group were significantly lower than those in control group; GSH content and GSH-Px activity of astrocytes in 0.5 mmol / L LaCl3 group were significantly lower than those in control group and 0.25 mmol / L LaCl3 group; GSH-Px activity and GSH-Px activity in astrocytes of 1.0 mmol / L NaCl group decreased to 66% and 43% of the control group LaCl3 group. In addition, the content of MDA in astrocytes in each LaCl3 group was significantly higher than that in the control group, and increased with the dose of LaCl3. Conclusion LaCl3 can damage astrocytes, which may be related to the oxidative damage of astrocytes induced by LaCl3.