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华蟾素注射液(原称抗癌注射液),是蟾蜍全皮的水溶性成分为主的医用制剂.本文作如下研究:1.免疫作用:①本品对小鼠每天腹腔注射连续给药14天后,可导致白细胞总数升高(P<0.01),而T淋巴细胞百分比的升高则不显著(P>0.05).②本品可使小鼠的血清IgG测定值由9.44±1.25mg/ml((?)±SE)上升到11.38±0.41mg/ml,有显著意义(P<0.01).③对家兔连续给本品14天,可使其抗“H”凝集效价测定达到1:1280,证实可显著地提高体液免疫作用(P<0.01).2.合并用抗癌药时的影响:①给小鼠皮下注射本品20ml/kg,并分别合用CTX400mg/kg、5-FU500mg/kg、MC8mg/kg腹腔注射,均每天一次,连续5天,逐日计算其平均存活百分率,如此重复4次,结果均未见有意义的变化.②通过三种不同的组合方式,联合给于小鼠华蟾素注射液(5ml/kg)腹腔注射,和CTX(100mg/kg)皮下注射,然后分别测定其白细胞总数和T淋巴细胞百分比,半数溶血值(CH50)和血清IgG的变化,结果均提示本品对用CTX引起的免疫功能抑制有一定对抗性的影响.3.亚急性毒性试验:对大鼠采用本品两组剂量(20ml/kg,4ml/kg)腹腔注射,隔日一次给药,进行亚急性毒性观察.结果发现两组剂量均对大鼠体重增长产生抑制作用,应予以注意.
Cinobufotalin injection (formerly known as anti-cancer injection) is a water soluble component of toad skin-based medical preparations.This paper is as follows: 1. Immunity: ① The product of daily intraperitoneal injection of mice continuous administration After 14 days, the total number of leukocytes increased (P <0.01), while the percentage of T lymphocytes increased not significantly (P> 0.05) .②The serum IgG of the mice increased from 9.44 ± 1.25mg / ml ((±) ± SE) to 11.38 ± 0.41mg / ml, which was significant (P <0.01) .③The rabbits were given this product for 14 consecutive days and the anti-“H” agglutination titer reached 1 : 1280, confirmed that can significantly improve the humoral immunity (P <0.01) .2 when combined with anticancer drugs: ① subcutaneous injection of the goods 20ml / kg, and were combined CTX400mg / kg, 5-FU500mg kg / kg and MC8mg / kg intraperitoneally, were given once a day for 5 consecutive days to calculate the average daily survival percentage, so repeated 4 times, the results showed no significant changes.②Through three different combinations, given in combination The mice were injected intraperitoneally with cinobufacini injection (5ml / kg) and subcutaneously with CTX (100mg / kg). The total white blood cell count, T lymphocyte percentage, CH50 and Clear IgG changes, the results suggest that the product of CTX-induced immune function inhibition has a certain confrontational effect .3 subacute toxicity test: The two groups of rats using the dose (20ml / kg, 4ml / kg) Intraperitoneal injection, once every other day, subacute toxicity observed.The results showed that the two groups of doses have an inhibitory effect on body weight gain, should be noted.