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目的 分析比较用注射器穿刺法和真空采血针穿刺法采集血培养标本的采血效果.方法采用回顾性调查方法,对本院两年间采用两种不同血培养采集方法效果进行比较,对送检后的血培养标本结果进行分析,比较两种血培养采集方法在标本污染率、穿刺成功率、皮肤破损、血细胞破坏等方面的发生情况.结果1584份送检的血培养标本中,真空采血针组较注射器穿刺组平均采血时间短、一次穿刺成功率高、针头滴血及皮下瘀斑少、手污染率低;标本溶血率、凝血、血小板破坏率均较低,两组比较差异有统计学意义(P<0.05).结论真空采血针组血培养污染率低,穿刺成功率高,痛苦小,建议临床采用真空采血针抽取血培养标本.“,”objective To compare and analyze two blood culture sampling method with syringe puncture method and the vacuum blood collection needle puncture method.Methods Retrospective survey effect of the two kinds of different blood culture sampling methods in two years in our hospital.Specimen pollution rate,success rate of puncture,broken rate of punctured skin and damage rate of blood cell,etc.were compared.Results Of 1584 specimens of blood culture, the collection time of the vacuum blood collection needle group was shorter than the syringe puncture group, higher success rate of puncture,lower needle drop rate,subcutaneous spot rate,hand contamination rate and blood destruction rate.(P<0.05). Conclusion Vacuum blood collection needle group had lower contamination rate, higher success puncture rate,smaller pain,is preferred for clinical use.