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目的 探讨大鼠脑缺血２ ｈ 后不同时间再灌注脑组织碱性成纤维细胞生长因子（ｂＦＧＦ）的表达程度。方法 采用线栓法栓堵一侧大鼠大脑中动脉，建立脑缺血及再灌注模型。用免疫组化的方法，对缺血脑组织ｂＦＧＦ的表达进行观察。用统计学分析ｂＦＧＦ阳性细胞的数据。结果 正常脑组织中只有微量ｂＦＧＦ的表达；脑缺血２ ｈ 后再灌注０．５ ｈ 起，ｂＦＧＦ少量表达，为神经元表达；脑缺血２ ｈ 后再灌注２２ ｈ，ｂＦＧＦ表达达到高峰，神经元及神经胶质细胞均有表达。脑缺血２ ｈ 后再灌注１６６ ｈ，ｂＦＧＦ仍有持续表达。结论 ｂＦＧＦ参与缺血脑组织的修复过程
Objective To investigate the expression of basic fibroblast growth factor (bFGF) in rat brain after reperfusion for 2 h at different time points. Methods Thread occlusion was used to occlude the middle cerebral artery of rats and the model of cerebral ischemia and reperfusion was established. The expression of bFGF in ischemic brain tissue was observed by immunohistochemistry. Data for bFGF-positive cells were statistically analyzed. Results There was only trace amount of bFGF expression in normal brain tissue. Expression of bFGF was detected in small amount at 2 h after cerebral ischemia and 0.5 h after reperfusion. Expression of bFGF peaked at 2 h after cerebral ischemia and reperfusion for 22 h, Both neurons and glial cells are expressed. Two hours after cerebral ischemia, reperfusion for 166 h, bFGF continued to be expressed. Conclusion bFGF participates in the repair process of ischemic brain tissue