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目的:将合成的两种量子点应用于研究人骨肉瘤HOS细胞系,初步检测其生物毒性,以确定本研究所制备量子点可否应用于骨肉瘤的基础研究。方法:将生长良好的HOS细胞与制备的两种量子点分别共培养,使用MTT试剂盒检测不同时间点细胞活性,实验进行三次,取其平均值,分析所得数据,并绘制出量子点浓度-细胞活性曲线,分析得出两者之间的量效关系。结果:1.4μM的CdTe/ZnS QDs和0.275μM的Cd Te/Cd S QDs分别是本实验中对HOS细胞的最高毒性浓度。较短时间(30 min)的暴露分别导致了48.6±0.9%和31.9±0.8%的细胞死亡,3 h后分别有33.7±2.3%和49.4±1.1%的细胞存活。而在孵育了18 h之后,只有28.0±1.6%和15.3±1.6%的细胞存活。我们可以观察到均为典型的时间/浓度曲线。结论:选用适宜浓度以及共培养时间,本实验制备的量子点完全可以满足纳米量子点粒子使用于HOS细胞研究的基本生物学条件,可以进行人骨肉瘤HOS细胞测温等的一些列实验操作。由于量子点自身优越的光学性能以及可接受的生物安全性,在肿瘤研究领域具有很大的潜力,将会成为肿瘤示踪、检测、以及靶向治疗新的有力工具。
OBJECTIVE: To investigate the biological toxicity of two kinds of quantum dots synthesized in human osteosarcoma HOS cell line to determine whether the quantum dots prepared in this study can be used in the basic research of osteosarcoma. Methods: The well-growing HOS cells were co-cultured with the prepared two kinds of quantum dots respectively. MTT kit was used to detect the cell viability at different time points. The experiment was performed three times, the average value was taken, the data were analyzed, and the quantum dot concentration- Cell activity curve analysis obtained the dose-effect relationship between the two. Results: 1.4 μM CdTe / ZnS QDs and 0.275 μM Cd Te / Cd S QDs were the highest toxic concentrations of HOS cells in this experiment, respectively. Exposure to a shorter time (30 min) resulted in cell death of 48.6 ± 0.9% and 31.9 ± 0.8%, respectively, and 33.7 ± 2.3% and 49.4 ± 1.1% of cell survival after 3 h, respectively. After 18 h of incubation, only 28.0 ± 1.6% and 15.3 ± 1.6% of the cells survived. We can observe that all are typical time / concentration curves. Conclusion: The appropriate concentration and time of co-culture can be used to prepare the quantum dots. The quantum dots prepared in this experiment can fully meet the basic biological conditions for the use of nano-QDs in the study of HOS cells, and can perform some experimental operations such as temperature measurement of HOS cells in human osteosarcoma. Because of their superior optical properties and acceptable biosafety, quantum dots have great potential in cancer research and will become powerful new tools for tumor tracing, detection and targeted therapy.