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目的研究纤维蛋白黏合剂的潜在免疫原性。方法建立间接ELISA法检测抗体条件;新西兰白兔创伤实验及Balb/c小鼠实验考察纤维蛋白黏合剂的免疫原性。结果新西兰白兔创伤实验中,给药一周后兔体内即可检测出抗体,给药2周后抗体水平明显升高,6周后开始下降。各组血清滴度均呈现先上升后下降的趋势;小鼠实验中,纤维蛋白黏合剂作用于小鼠后,高、中、低各剂量组均能够激活小鼠的免疫系统,增强嗜中性粒细胞的吞噬功能;增加小鼠免疫器官重量及脾细胞的转化能力。结论研究表明,纤维蛋白黏合剂对于兔诱发特异性抗体,对小鼠表现出明显的免疫原性。
Objective To investigate the potential immunogenicity of fibrin glue. Methods The antibody conditions were established by indirect ELISA. The immunogenicity of fibrin glue was investigated in New Zealand white rabbits and Balb / c mice. Results In New Zealand white rabbits, the antibodies were detected in rabbits one week after administration. After 2 weeks of administration, the antibodies were significantly increased and began to decline after 6 weeks. The serum titer of all groups showed the trend of first rising and then decreasing. In the experiment of mice, the fibrin glue acts on the mice, and the high, medium and low doses of each group can activate the immune system and enhance the neutrophilic Phagocytosis of granulocytes; increase the weight of immune organs and the transforming ability of spleen cells in mice. Conclusions Studies have shown that fibrin glue induces specific antibodies to rabbits and shows significant immunogenicity in mice.