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用快速、恒压的无钙和胶原酶Ｔｙｒｏｄｅ液相继灌流豚鼠心脏冠脉系统后，再经无钙液室温浸泡心脏和用改变的Ｋ－Ｂ液帮助分离细胞的恢复，可获得耐钙的游离心肌细胞。全细胞电流记录：静息电位为－７２±９ｍＶ（ｎ＝１２），并显示出快内向电流（ＩＮａ），可被异搏定阻断的慢钙离子流和时间依赖性外向钾流（Ｉｋ）；单通道记录分别显示了Ｎａ＋Ｃａ２＋和Ｋ＋通道的电压依赖性等特征。结果表明了用此法分离的细胞具有耐钙性和正常电生理特性。
Fast, constant pressure of calcium-free and collagenase Tyrode liquid guinea pig heart after sequential perfusion of the coronary system, and then by calcium-free solution at room temperature soaking the heart and with the change of K-B solution to help separate the recovery of cells can be resistant to calcium free Cardiomyocytes. Whole-cell current recordings: resting potential of -72 ± 9 mV (n = 12) and showed a rapid inward current (INa), a slow calcium ion current blockable by verapamil, and a time-dependent outward potassium current ); Single-channel recording shows the voltage-dependent characteristics of Na + Ca2 + and K + channels, respectively. The results show that cells isolated by this method have calcium resistance and normal electrophysiological properties.