论文部分内容阅读
目的开颅电凝法制作大鼠大脑中动脉(Middle Cerebral Artery MCA)远端闭塞模型,观察实验大鼠相关行为学变化,TTC染色证实梗死范围。方法鼠随机分为实验组和对照组各12只,术前3天开始行为学测试。实验组暴露左侧MCA,将其在大脑下静脉及嗅束之间电凝闭塞;对照组只暴露不电凝。术后24h记录大鼠行为学改变及评分,断头取脑进行TTC染色。结果实验组12只大鼠有11只造模成功,Longa评分2分9只,3分2只,未出现进食困难及颞下颌关节紊乱等并发症。对照组12只大鼠术后无神经功能障碍及行为学异常。术后24h行为学测试:转棒试验,实验组大鼠滚轴上平均停留时间(32.64s±4.30s)短于对照组(160.92s±5.76s);平衡木试验,实验组平均得分(2.82±0.60)低于对照组(6.00±0.00);悬空旋转试验,实验组大鼠向右侧摇摆所占比例(92.73%±5.79%)高于对照组(50.83%±3.24%);以上差异均具有统计学意义(P<0.05)。TTC染色提示造模梗死部位累及运动皮层在内的大脑皮层背外侧部。结论电凝闭塞MCA远端制作局灶脑缺血模型,获得梗死面积一致,伴对侧肢体运动障碍,死亡率低,模型稳定可靠。
Objective To establish a model of distal occlusion of middle cerebral artery (MCA) in rats by electrocution of the craniotomy and observe the changes of behavioral changes in experimental rats. TTC staining confirmed the infarct size. Methods The rats were randomly divided into experimental group and control group, each with 12 rats. Behavioral tests began 3 days before the operation. In the experimental group, the left MCA was exposed and occluded between the inferior vena cava and the olfactory tract. The control group was exposed without electrocoagulation. Behavioral changes and scores were recorded 24 hours after operation, and the brain was decapitated for TTC staining. Results In the experimental group, 11 rats were successfully established. The Longa score was 2: 9 and 3: 2. No complications such as eating difficulty and temporomandibular joint disorder occurred. Twelve rats in the control group had no neurological dysfunction and behavioral abnormalities after operation. The behavioral test 24h after operation: the bar rotation test, the average retention time (32.64s ± 4.30s) in the experimental group was shorter than that in the control group (160.92s ± 5.76s); the average score in the experimental group was 2.82 ± 0.60) was lower than that of the control group (6.00 ± 0.00). The dangling rotation test showed that the percentage of rats to the right side of the experimental group (92.73% ± 5.79%) was higher than that of the control group (50.83% ± 3.24% Statistical significance (P <0.05). TTC staining prompted the modeling of the infarct involved the motor cortex, including the dorsolateral part of the cortex. Conclusion MCI distal focal model of focal cerebral ischemia produced infarction area consistent with the contralateral limb dyskinesia, mortality is low, the model is stable and reliable.