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目的 探讨HDV/HBV感染树鼩肝组织中ICE表达与HDV感染之间的关系,以及ICE在丁型肝炎肝细胞凋亡中的作用。 方法 采用免疫组化技术对45份HDV感染树鼩肝组织中HDVAg,ICE的表达进行了检测;应用原位末端标记技术对肝组织凋亡进行检测;并应用免疫组化双重染色对HDVAg,ICE的表达以及肝细胞凋亡进行了检测。 结果 肝组织45份中有43份可检出ICE(阳性率96％),有41份可检出凋亡细胞(阳性率91％),ICE在肝细胞浆和(或)膜上表达,以肝细胞浆内表达为主,HDVAg表达与ICE表达之间有显著相关性X~2=36.2(P<0.05),HDVAg表达越强,ICE表达越强,凋亡阳性细胞在肝细胞索多呈散在分布,在坏死灶中也可检出,凋亡在HDVAg阳性和阴性细胞中均可发生,以在HDVAg阳性细胞内发生为主,ICE表达与肝细胞凋亡之间有显著相关性X~2=35.6(P<0.05),ICE表达越强,凋亡阳性细胞越多。 结论 丁型肝炎病毒感染和未感染的肝细胞均可发生凋亡,但凋亡只在少数细胞内发生;HDV可诱导ICE表达,ICE在肝细胞凋亡中起重要作用。
Objective To investigate the relationship between ICE expression in HDV / HBV infected tree shrew liver tissue and HDV infection and the role of ICE in hepatocyte apoptosis in hepatitis D virus. Methods The expressions of HDVAg and ICE in 45 liver cirrhosis tissues infected with HDV were detected by immunohistochemistry. The apoptosis of hepatic tissue was detected by in situ end labeling technique. The expressions of HDVAg, ICE The expression of hepatocytes and apoptosis of hepatocytes were detected. Results ICE was detected in 43 out of 45 liver tissues (96% positive), 41 were apoptotic cells (91% positive), and ICE was expressed in hepatocyte plasma and / or There was a significant correlation between HDVAg expression and ICE expression (X ~ 2 = 36.2, P <0.05). The stronger the expression of HDVAg, the stronger the expression of ICE and the higher the number of apoptotic cells in hepatocytes Scattered in the necrotic lesions can also be detected, apoptosis in HDVAg positive and negative cells can occur to occur in HDVAg positive cells, ICE expression and hepatocyte apoptosis was significantly related to X ~ 2 = 35.6 (P <0.05). The stronger ICE expression, the more apoptosis-positive cells. Conclusions Apoptosis can occur in both HepG2 infected and uninfected Hepatitis B cells. However, apoptosis occurs only in a few cells. HDV can induce ICE expression, and ICE plays an important role in hepatocyte apoptosis.