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目的 检测人肝细胞癌（ＨＣＣ）组织中丙型肝炎病毒（ＨＣＶ）基因型。方法 用ＲＴＰＣＲ法常规检测ＨＣＣ组织和部分患者血清ＨＣＶ，再以巢式ＲＴＰＣＲ法对ＨＣＶ阳性标本中５种ＨＣＶ基因型进行鉴别。对多数标本中ＨＢｓＡｇ和ＨＢＶＤＮＡ也进行了免疫组化和原位杂交检测。结果 ＨＣＣ组织中ＨＣＶ阳性率为５０％（３９／７８），与ＨＢＶ者（６４．４％）差异无显著性意义（Ｐ＞０．０５），两者重叠感染率３４％（２０／５９）。在１０例血清ＨＣＶ阴性ＨＣＣ患者中，３例ＨＣＣ冰冻组织检出ＨＣＶＲＮＡ。在ＨＣＣ组织中共检出３种ＨＣＶ基因型，其中２ａ、１ｂ、３ａ和混合型分别为１７例（４３．６％）、９例（２３．１％）、４例（１０．３％）和２例（５．１％），各基因型间差异有极显著性意义（Ｐ＜０．０１），但２ａ型与１ｂ型间差异无显著性（Ｐ＞０．０５），２ａ型与ＨＢＶ的重叠感染明显较其他ＨＣＶ基因型者常见（Ｐ＜０．０５）。结论 ２ａ型ＨＣＶ是ＨＣＣ组织中最常见的基因型并易与ＨＢＶ重叠感染率，ＨＣＣ患者血清ＨＣＶ检测不一定能准确反映ＨＣＣ组织中的ＨＣＶ感染率，其所示基因型也有所不同。
Objective To detect the hepatitis C virus (HCV) genotypes in human hepatocellular carcinoma (HCC) tissues. Methods Serum HCV of HCC tissues and some patients were routinely detected by RT PCR method. Five HCV genotypes in HCV positive specimens were identified by nested RT PCR method. Most specimens of HBsAg and HBVDNA also carried out by immunohistochemistry and in situ hybridization detection. Results The positive rate of HCV in HCC tissues was 50% (39/78), but no significant difference with that in HBV (64.4%) (P> 0.05). The overlapping infection rate was 34% (20/59) . In 10 cases of serum HCV-negative HCC patients, 3 cases of HCC frozen tissue detected HCVRNA. Three HCV genotypes were detected in HCC tissues, including 17 (43.6%), 9 (23.1%), 4 (10.3%) and There was no significant difference between type 2a and type 1b (P> 0.05), type 2a and HBV Of overlapping infections were significantly more common than other HCV genotypes (P <0.05). Conclusions Type 2a HCV is the most common genotype in HCC tissues and is easily overlap with HBV infection. The detection of serum HCV in HCC patients may not accurately reflect the HCV infection rate in HCC tissues, and the genotypes indicated are different.